Stem Cell Thesis

Due to its ability to allow complete cell removal, the smaller void size scaffold was used to develop a method for long-term PSC culture.This, however, resulted in gradual cell differentiation, suggesting that a mixed-topography was more suited for PSC growth.

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Cardiovascular diseases (CVD) are the leading cause of death in developed societies.

Vascular stem cells (VSCs) have been proposed as a cell population with regenerative potential for CVD.

This is in part likely to be a result of adaptation to the artificial culture environment, known to force cell structural away from the native state, resulting in altered gene expression.

The mechanisms by which PSCs, specifically, integrate cues from their physical microenvironment remain largely unexplored.

The mechanisms of mechanotransduction in PSCs are poorly understood; the culture system developed here allowed for a direct comparison between two-dimensional (2D, conventional) and three-dimensional (3D) culture with substrate geometry as the only variable.

The structure of the actin cytoskeleton, as well as that of intermediate filaments and microtubules, was less-developed after 3D culture.

The results demonstrated that, after 5 days of culture, the average number of neurospheres in the cultured ts NSCs was significantly lower compared with r NSCs (P=0.0031).

Additionally, compared with the r NSCs, ts NSCs exhibited an enhanced differentiation ability towards neurons.

Alvetex® Strata has been previously shown to be a suitable substrate for the long-term maintenance of PSCs for enhanced differentiation (up to approximately 50 days).

Here, we show that the same effect is possible from a single 10-day conditioning step, we term “priming”, with evidence of enhanced differentiation in vitro and in vivo.


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